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Visualization of read quality profiles plot - thin heatmap

Status: Open.

#2178 In benjjneb/dada2;

· mmladinic opened on Feb 11, 2026

Mismatched R1/R2 PAIRS FOUND (these will crash filterAndTrim)

Status: Open.

#2177 In benjjneb/dada2;

· Kumaratenna opened on Feb 5, 2026

Issue with DADA2: Low ASV numbers in PacBio full-length ITS data

Status: Open.

#2175 In benjjneb/dada2;

· cherry52HZ opened on Feb 5, 2026

Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?

Status: Open.

#2173 In benjjneb/dada2;

· wanghang7410 opened on Jan 29, 2026

cannot remove primers on reverse reads using cutadapt

Status: Open.

#2172 In benjjneb/dada2;

· kdbchau opened on Jan 8, 2026

error in dada2 - error rates could not be estimated

Status: Open.

#2171 In benjjneb/dada2;

· lejlapasic-ctrl opened on Dec 29, 2025

Discrepancy between number of ASVs and taxonomic assignments

Status: Open.

#2170 In benjjneb/dada2;

· judithsantano opened on Dec 16, 2025

Simple mapping of original read IDs (Headers) to final DADA2 ASVs for validation purposes

Status: Open.

#2168 In benjjneb/dada2;

· CassandraHjo opened on Dec 15, 2025

DADA2 for full length ITS (PacBio)

Status: Open.

#2167 In benjjneb/dada2;

· laurarcasillas opened on Dec 10, 2025

Handling ITS reads with variable lengths: Should ASVs be normalized to avoid redundancy?

Status: Open.

#2166 In benjjneb/dada2;

· CarolTMartins opened on Dec 5, 2025

removeBimeraDenovo freezing then crashing

Status: Open.

#2165 In benjjneb/dada2;

· damianasrojas opened on Nov 20, 2025

[HELP] All ASVs occurring only once across samples - 16S amplicon

Status: Open.

#2164 In benjjneb/dada2;

· Ka1wenSun opened on Nov 13, 2025