Experimental data on pheromone characterization of Spodoptera Picta from the article:
Odorant receptor structures predict the major female sex pheromone component in a moth. A. Comte, Z. Wei, H. Li, W.Z. Xing, R. Moracci, J. Zhang*, S. Fiorucci*, E. Jacquin-Joly*. submitted.
corresponding authors: jinzhang001@njau.edu.cn, sebastien.fiorucci@univ-cotedazur.fr, emmanuelle.joly@inrae.fr
This README file describes the structure and contents of the Excel file that compiles the analysis of all single sensillum recording (SSR) data used in the study "Odorant receptor structures predict the major female sex pheromone component in moth". In this study, we investigated the responses of two predicted pheromone receptors of Spodoptera picta—SpicOR5 and SpicOR75—to a panel of diverse moth female sex pheromone components. The two studied odorant receptors were heterologously expressed in Drosophila melanogaster, and SSR was used to record olfactory sensory neuron (OSN) activity. An initial screen of the full odorant panel was performed for each receptor to define its specificity. Then, dose-response experiments were conducted for the newly identified ligands to quantify receptor sensitivity.
The file is composed of 4 sheets:
Screen panel OR5 : Analysis of OSN activity in neurons expressing SpicOR5 following stimulation with a panel of 26 moth female sex pheromone components. Neuronal responses were recorded from eight transgenic flies.
Dose response OR5 : Analysis of OSN activity in neurons expressing SpicOR5 following stimulation of newly identified receptor ligand at 6 different concentrations. Neuronal responses were recorded from eight transgenic flies.
Screen panel OR75 : Analysis of OSN activity in neurons expressing SpicOR75 following stimulation with a panel of 26 moth female sex pheromone components. Neuronal responses were recorded from eight transgenic flies.
Dose response OR75 : Analysis of OSN activity in neurons expressing SpicOR75 following stimulation of newly identified receptor ligands at 6 different concentrations. Neuronal responses were recorded from eight transgenic flies.
To evaluate OSN responses to each tested odorant or concentration, the spontaneous firing rate was subtracted from the firing rate measured during odorant stimulation. Responses are reported in spikes per second.