Antibiotics

Below is a list of antibiotics used in the lab for culturing/selecting for E. coli variants. The following table gives the stock and working concentrations along with any useful info. Below the table is more detailed information about each antibiotic

Concentrations

All stocks should be stored at -20° C. This table should be taken as a quick reference. See each sub section for more details.

Antibiotic	Stock Concentration	Working Concentration	Vendor	Notes
Ampicillin	100 mg / mL	100 µg / mL	Sigma-Aldrich A9518-5G	Dissolve in $ddH_2O$
Carbenicillin	100 mg / mL	100 µg / mL	Fisher BP2648250	Dissolve in $ddH_2O$
Kanamycin	100 mg / mL	100 µg / mL	Millipore-Sigma K1377	Dissolve in $ddH_2O$
Chloramphenicol	25 mg / mL	25 µg / mL	Millipore-Sigma C1919-25G	Dissolve in Ethanol
Spectinomycin	50 mg / mL	50 µg / mL	Sigma-Aldrich S4014	Dissolve in $ddH_2O$
Gentamicin	10 mg / mL	10 µg / mL	Millipore-Sigma G1264-1G	Dissolve in $ddH_2O$
Tetracycline	10 mg / mL	10 µg / mL	Sigma-Aldrich T7660-5G	Dissolve in $ddH_2O$

More Information

Ampicillin & Carbenicillin

Kanamycin

Chloramphenicol

Spectinomycin

Gentamycin

Tetracycline