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# Overview of `junctions extract` command
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The `junctions extract` command can be used to extract exon-exon junctions from an RNAseq BAM file. The output is a BED file in the BED12 format. We have tested this command with alignments from TopHat and by comparing the exon-exon junctions with the `junctions.bed` file produced from TopHat.
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The `junctions extract` command can be used to extract exon-exon junctions from an RNAseq BAM file. The output is a BED file in the BED12 format. We have tested this command with alignments from HISAT2, TopHat2, STAR, kallisto, and minimap2 and by comparing the exon-exon junctions with the `junctions.bed` file produced from TopHat.
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