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218e194
Incorporated changes from ampicon_add_runsheet_visualizations
torres-alexis Feb 8, 2024
ceba9d8
GL-DPPD-7104-B.md updates
torres-alexis Feb 8, 2024
78ff717
README Text change/fix
torres-alexis Feb 8, 2024
de73f30
Added _GLAmpSeq file suffix to SW_AmpIllumina_B plots
torres-alexis Feb 8, 2024
922adee
Added assay suffixes to SW_AmpIllumina-B Snakefile and R-visualizatio…
torres-alexis Feb 8, 2024
252b36f
Typo fix
torres-alexis Feb 8, 2024
6466384
SW_AmpIllumina-B R visualization command fix
torres-alexis Feb 8, 2024
eef06f2
Merge pull request #56 from torres-alexis/amp_pr
asaravia-butler Feb 8, 2024
00718b3
Pointing to the updated pipeline (version B) as the current one
asaravia-butler Feb 8, 2024
14e9938
typo fix
asaravia-butler Feb 9, 2024
b71314e
Fixing broken link
asaravia-butler Feb 9, 2024
391dd3b
SW_AmpIllumina-B: Added primer check to run_workflow.py
torres-alexis Feb 12, 2024
54746ba
SW_AmpIllumina-B: 16S and ITS removal changes
torres-alexis Feb 13, 2024
9f80d0c
Added uniqueness check to run_workflow primer validation
torres-alexis Feb 13, 2024
ce79021
Merge pull request #58 from torres-alexis/SW_AmpIllumina-B_primer_check
asaravia-butler Feb 23, 2024
786fcbd
Adding links to mouse heart and liver gene marker files
asaravia-butler Mar 6, 2024
fe5e366
SW_AmpIllumina-B: Optional visualizations code
torres-alexis Mar 7, 2024
091bed8
SW_AmpIllumina-B: visualizations argument fix
torres-alexis Mar 8, 2024
eac35ce
SW_AmpIllumina-B: Updated version references in READMEs from 1.2.1 to…
torres-alexis Mar 11, 2024
2051ddb
SW_AmpIllumina-B: Added 1.2.2 info to changelog
torres-alexis Mar 11, 2024
2aa92ab
SW_AmpIllumina-B: Added --visualizations to readme
torres-alexis Mar 11, 2024
8d82d81
Merge pull request #66 from torres-alexis/SW_AmpIllumina-B-optional-vis
asaravia-butler Mar 15, 2024
bea7dc3
Typo fix
asaravia-butler Mar 15, 2024
dd75cc8
Fix incorrect link
asaravia-butler Mar 15, 2024
08eebfa
Add enable_visualizations to config
torres-alexis Mar 15, 2024
792c347
Fixed run_workflow for single runsheet OSDR datasets
torres-alexis Mar 15, 2024
2e1b7c9
SW_AmpIllumina-B: Updated dendrogram label scaling
torres-alexis Mar 16, 2024
c77488e
SW_AmpIllumina-B: Visualization script fix
torres-alexis Mar 16, 2024
b3af487
SW_AmpIllumina-B:Improve manual vis script comment
torres-alexis Mar 18, 2024
e0916b9
SW_AmpIllumina-B: Start manual visualizations docs
torres-alexis Mar 18, 2024
9b2630c
SW_AmpIllumina-B: Add visualizations/README.md
torres-alexis Mar 18, 2024
a7afaba
SW_AmpIllumina-B: Typo fix
torres-alexis Mar 18, 2024
75afc78
SW_AmpIllumina-B: Typo fix
torres-alexis Mar 18, 2024
0b98617
SW_AmpIllumina-B/: Typo fix
torres-alexis Mar 19, 2024
daaf1a0
SW_AmpIllumina-B: Typo fix
torres-alexis Mar 19, 2024
86b2db5
SW_AmpIllumina-B: Rscript execution readme updates
torres-alexis Mar 19, 2024
d961d02
SW_AmpIllumina-B: Typo fix
torres-alexis Mar 19, 2024
9f03919
Merge pull request #68 from torres-alexis/Amp-B-config-typo
asaravia-butler Mar 19, 2024
35975a6
Fixing typos, broken links, and adding additional instructions.
asaravia-butler Mar 19, 2024
9936a7a
SW_AmpIllumina-B: Vis script readme updates, fixes
torres-alexis Mar 19, 2024
d4b8190
SW_AmpIllumina-B: Vis Readme updates
torres-alexis Mar 19, 2024
21b03aa
SW_AmpIllumina-B: vis readme update
torres-alexis Mar 19, 2024
1eb0870
SW_AmpIllumina-B: Add color legend to vis readme
torres-alexis Mar 19, 2024
a3e1bac
Merge pull request #69 from torres-alexis/DEV2_SW_AmpIllumina-B
asaravia-butler Mar 20, 2024
94d046a
Fixing download links
asaravia-butler Mar 20, 2024
fa6626b
SW_AmpIllumina-B: assay suffix changes
torres-alexis Mar 20, 2024
b8b9be7
Merge pull request #70 from torres-alexis/SW_AmpIllumina-B-suffix-fix
asaravia-butler Mar 20, 2024
2724b88
SW_AmpIllumina-B: GL-DPPD-7104-B.md typo fixes
torres-alexis Mar 20, 2024
e7f9bed
Merge pull request #71 from torres-alexis/SW_AmpIllumina-B-doc
asaravia-butler Mar 20, 2024
4f53b7f
SW_AmpIllumina-B: Update changelog
torres-alexis Mar 20, 2024
6bf6144
SW_AmpIllumina-B: changelog update
torres-alexis Mar 20, 2024
a3f50f4
Merge pull request #73 from torres-alexis/SW_AmpIllumina-B-doc
asaravia-butler Mar 20, 2024
38829da
Updating to correct genelab-utils version
asaravia-butler Apr 18, 2024
6193ae6
SW_AmpIllumina-B: --target documentation changes
torres-alexis Apr 18, 2024
3fc4b79
Merge pull request #81 from torres-alexis/DEV2_SW_AmpIllumina-B
asaravia-butler Apr 18, 2024
319aae5
first commit
olabiyi May 1, 2024
b78bd10
fixed incomplete download issue
olabiyi May 6, 2024
0981b91
Added help
olabiyi May 11, 2024
0b779ec
Fixed pipeline name
olabiyi May 11, 2024
9fc0765
Added figshare links and documentation
olabiyi May 16, 2024
0a568d7
replaced create.py with old working version
olabiyi May 17, 2024
6809a29
Added queue size and formatted help
olabiyi May 17, 2024
32e7f50
Added profile to log info
olabiyi May 20, 2024
522e347
Fixed help variable
olabiyi May 20, 2024
3eb1876
Added software version tracking
olabiyi Jun 25, 2024
9e25711
Added README.md
olabiyi Jun 27, 2024
286e3da
Added README.md
olabiyi Jun 27, 2024
95ea13e
Formatted nextflow.contig
olabiyi Jun 28, 2024
b3296da
Added help variable
olabiyi Jul 8, 2024
35809fb
Increased internet download timeout
olabiyi Jul 9, 2024
1ad724a
Fixed missing required output issue
olabiyi Jul 16, 2024
4fb45e0
updated README created Nextflow directory
olabiyi Aug 30, 2024
4169e07
Fixed README typo
olabiyi Aug 30, 2024
0f3e1a0
Added post-processing scripts
olabiyi Sep 12, 2024
d1bcb32
Fixed Typos
olabiyi Sep 13, 2024
95e1f70
added missing script
olabiyi Sep 27, 2024
e7398cd
Added ANCOMBC
olabiyi Oct 26, 2024
a8bf074
Fixed typo
olabiyi Oct 26, 2024
565a0c6
Add ANCOM environment definition
olabiyi Oct 26, 2024
036b1bd
Fixed Rscript run method
olabiyi Oct 29, 2024
4a94084
Increased ANCOMBC's default memory requirement
olabiyi Oct 29, 2024
e879110
Refactored diversity and differential abundance testing
olabiyi Nov 14, 2024
1716f14
Merge pull request #102 from olabiyi/DEV_Amplicon_Illumina_NF_conversion
asaravia-butler Nov 15, 2024
57b5bc0
Added chloroplast and mitochondria filtering
olabiyi Nov 15, 2024
1dbfe66
Deleted R here package usage
olabiyi Nov 15, 2024
49ce1aa
Merge pull request #132 from olabiyi/DEV_Amplicon_Illumina_NF_conversion
asaravia-butler Nov 15, 2024
1702918
Fixed group means and stds
olabiyi Nov 18, 2024
8a7bbaa
set ANCOMBC image to 2.6.0
olabiyi Nov 18, 2024
a4d1505
added run workflow script
olabiyi Nov 27, 2024
7a97404
Edited README
olabiyi Nov 27, 2024
f7df02f
Edited README
olabiyi Nov 27, 2024
addf4d6
Fixed no extra argument to nextflow bug
olabiyi Nov 28, 2024
7a9c637
updated UNITE_DB link
olabiyi Dec 5, 2024
10afc84
added deseq
olabiyi Dec 24, 2024
b8ea788
Fixed beta diversity
olabiyi Dec 24, 2024
51bdac9
Updated pipeline document
olabiyi Jan 2, 2025
0f68848
Added more comments to pipeline document
olabiyi Jan 3, 2025
d1670ff
Fixed taxize's api rate error
olabiyi Jan 6, 2025
e579cba
fixed post processing bug
olabiyi Jan 6, 2025
e48589a
Added remove rare functionality
olabiyi Jan 8, 2025
b61a9b5
updated nextflow version
olabiyi Jan 8, 2025
1998ed2
Fixed reads count column bug
olabiyi Jan 8, 2025
e11e03f
Updated readme and variables
olabiyi Jan 9, 2025
4c44697
Fixed software versions redundancy bug
olabiyi Jan 10, 2025
39bd0f2
Added diversity and differential abundance testing to README post-pro…
olabiyi Jan 13, 2025
409b6f8
updated pipeline document
olabiyi Jan 15, 2025
5b74691
updated pipeline document
olabiyi Jan 15, 2025
3657ba5
Documented functions
olabiyi Jan 18, 2025
7cc24dc
assigned a default value to params.help
olabiyi Jan 21, 2025
6923766
Added some more comments to preprocessing
olabiyi Jan 23, 2025
9a7726a
Formatting changes
bnovak32 Feb 21, 2025
8e94dc9
Added structural zeros removal functionality
olabiyi Feb 22, 2025
d53e371
Renamed volcano plots
olabiyi Feb 24, 2025
e5b6882
Merge pull request #1 from bnovak32/DEV_Amplicon_Illumina_NF_conversi…
olabiyi Feb 25, 2025
b707346
Updated the pipeline document
olabiyi Feb 27, 2025
483b939
Updated the pipeline document
olabiyi Feb 27, 2025
d48eb0f
Updated the pipeline document
olabiyi Feb 27, 2025
b202b79
Updated volcano plots file name
olabiyi Feb 28, 2025
e92025e
fixed minor bugs
olabiyi Feb 28, 2025
6c6bfef
Edited launchDir for post-processing
olabiyi Feb 28, 2025
ed05520
Update GL-DPPD-7104-B.md through step 6b plot_pcoa() function
asaravia-butler Mar 6, 2025
9a9beef
Updates through Step 6b - Read In Input tables
asaravia-butler Mar 7, 2025
f13ee57
Updated software versions
olabiyi Mar 9, 2025
89a1478
Updated software versions
olabiyi Mar 9, 2025
612c602
Updates through Step 7 through Rarefaction curve section of code block
asaravia-butler Mar 10, 2025
8da964e
fixed minor bugs
olabiyi Mar 10, 2025
22c9314
Fixed postprocessing multiqc raw count bug
olabiyi Mar 10, 2025
9a74553
Updated cutadapt.yaml
olabiyi Mar 11, 2025
838bfe3
Updates through the end of GL-DPPD-7104-B.md
asaravia-butler Mar 17, 2025
a9da151
Format fixes
asaravia-butler Mar 17, 2025
2ccef6c
Deleted numeric round ups
olabiyi Mar 19, 2025
97349a3
renamed pipeline doc
bnovak32 Mar 18, 2025
6d04866
restore previous B pipeline doc
bnovak32 Mar 18, 2025
52def98
updated numbering for section 6
bnovak32 Mar 18, 2025
ede943d
Updated beta diversity definitions
bnovak32 Mar 18, 2025
f5dd770
updated code intention in beta diversity
bnovak32 Mar 18, 2025
9cce1ec
Updated formatting
bnovak32 Mar 19, 2025
fc4c460
Renamed protocol id from B to C
olabiyi Mar 19, 2025
a5a69d6
Typo fixes and text updates for clarity
asaravia-butler Mar 19, 2025
722b7a1
Added color printing and software updates
olabiyi Mar 19, 2025
fe8d709
restored pipeline doc
olabiyi Mar 19, 2025
12eae50
Fixed debug message
olabiyi Mar 20, 2025
6bd498a
Added nextflow schema and renamed conda params
olabiyi Mar 20, 2025
4b8c6db
Added nextflow schema
olabiyi Mar 20, 2025
442f12d
Workflow can now be run from anywhere
olabiyi Mar 20, 2025
cb3afc5
made format changes to post-processing and edited run_workflow.py
olabiyi Mar 20, 2025
a46340a
made run_workflow.py runnable from anywhere
olabiyi Mar 20, 2025
7387d7e
made run_workflow.py runnable from anywhere
olabiyi Mar 20, 2025
f353bf4
Added function definitions
bnovak32 Mar 20, 2025
9228f5c
Fixed formatting and tested code blocks
bnovak32 Mar 21, 2025
898c710
minor additional edits
bnovak32 Mar 21, 2025
920c2fb
Merge pull request #3 from bnovak32/DEV_Amplicon_Illumina_NF_conversi…
olabiyi Mar 21, 2025
babe103
Generalized taxonomy plotting
olabiyi Mar 22, 2025
a830c91
Made formatting changes to main.nf
olabiyi Mar 22, 2025
7de7d3e
Handled NAs when calclating max, meann and sd
olabiyi Mar 23, 2025
d739c93
checked function parameters and return values
olabiyi Mar 24, 2025
6f9c69b
checked function parameters and return values
olabiyi Mar 24, 2025
da3ebc6
Merge pull request #133 from olabiyi/DEV_Amplicon_Illumina_NF_conversion
asaravia-butler Mar 24, 2025
8f30a0d
Added all updated references to changelog
asaravia-butler Mar 25, 2025
85ff26e
typo fix
asaravia-butler Mar 26, 2025
0288f3e
Adding note for DESeq2 parameters
asaravia-butler Mar 27, 2025
9b21e95
Added assement plots and edited content of processing_info.zip
olabiyi Apr 2, 2025
53f8c0b
Added launch script and edited pipeline document
olabiyi Apr 2, 2025
e910a47
Edit workflow launch scripts
olabiyi Apr 3, 2025
b55f1a9
deleted extra deseq2 line in diversity.yaml
olabiyi Apr 3, 2025
55aa3bc
Update README links
olabiyi Apr 7, 2025
9976d9c
boldened assessment plots output names
olabiyi Apr 24, 2025
7b55e7f
Update Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-…
olabiyi Apr 24, 2025
e7520e5
Update Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina-C/work…
olabiyi Apr 24, 2025
6931f30
Fixed broken links
olabiyi Apr 24, 2025
b741a80
Changed pipeline protocol in README from B to C
olabiyi Apr 24, 2025
6f7b7e5
Updated DA algorithm discussion
olabiyi Apr 24, 2025
1088bf2
Amplicon iIlumina NF conversion: Added launch scripts and diagnostic …
olabiyi Apr 25, 2025
318205d
Updating date
asaravia-butler May 2, 2025
0455fe1
Merge branch 'DEV_Amplicon_Illumina_NF_conversion' of https://github.…
olabiyi Jun 2, 2025
ff913a0
Fixed check.names and 2 groups bugs
olabiyi Jun 2, 2025
e418bf0
Modified clean-paths.sh
olabiyi Jun 2, 2025
107778f
Merge pull request #159 from olabiyi/DEV_Amplicon_Illumina_NF_conversion
asaravia-butler Jun 4, 2025
da0b88a
Point to latest Amplicon pipeline document in readme
torres-alexis Jun 10, 2025
11d34d3
Point to latest Amplicon pipeline document in readme
torres-alexis Jun 10, 2025
23a03cf
update vegan version in workflow readme
torres-alexis Jun 10, 2025
cd33e2d
Add initial changelog
torres-alexis Jun 10, 2025
1341458
update AmpIllumina workflow document
torres-alexis Jun 10, 2025
b50c30f
Rename NF_AmpIllumina-C/ folder to NF_AmpIllumina/
torres-alexis Jun 10, 2025
2f29587
Add June 2 edits to alpha, beta diversity scripts, taxonomy script in…
torres-alexis Jun 10, 2025
ef61b9a
update changelog
torres-alexis Jun 10, 2025
c4395f5
small fixes in workflow doc
torres-alexis Jun 10, 2025
5145d16
add dp_tools to listed software
torres-alexis Jun 10, 2025
5d1b29d
update dp tools to 1.3.8
torres-alexis Jun 10, 2025
81a9170
- Add sanitization for factor names with "-" to alpha diversity script
torres-alexis Jun 12, 2025
b700994
Change Alpha Diversity 0 groups error into warning + txt file
torres-alexis Jun 12, 2025
e650cb0
- Add try catches + error text outputs for alpha diversity, ancombc1,…
torres-alexis Jun 12, 2025
cba3e33
Add ancombc error text files to pipeline document
torres-alexis Jun 12, 2025
fc84241
update dp_tools container
torres-alexis Jun 13, 2025
7e41132
workflow updates to handle sparse datasets
asaravia-butler Jun 13, 2025
9bdb6ea
add subway plots, add to readmes
torres-alexis Jun 13, 2025
7b2efb2
Add Amplicon Workflow Diagram
asaravia-butler Jun 13, 2025
e044b00
Update README.md
asaravia-butler Jun 20, 2025
7beea8d
Typo fixes
asaravia-butler Jun 20, 2025
87e6a79
Adding runsheet specification
asaravia-butler Jun 20, 2025
b26ae78
Adding example PE runsheet
asaravia-butler Jun 20, 2025
3aef4aa
Adding example single-end runsheet
asaravia-butler Jun 20, 2025
789b889
Adding link to previous snakemake WF changes
asaravia-butler Jun 20, 2025
4d00d2d
Link fix
asaravia-butler Jun 20, 2025
28baa1b
removing example runsheet files from workflow_code dir
asaravia-butler Jun 20, 2025
c711f43
[NF-AmpIllumina] Update pipeline doc code
torres-alexis Jun 24, 2025
ef00761
re-add output prefix to warning text files
torres-alexis Jun 24, 2025
1e76e70
Fixes to handle 2-group datasets and rarefaction read depth
asaravia-butler Jun 24, 2025
a540de3
Add contrast table changes to AmpIllumina pipeline doc
torres-alexis Jun 25, 2025
3cf7a72
Add changes for retaining unassigned ASVs in DA analysis to NF-AmpIll…
torres-alexis Jun 25, 2025
cb89e03
Update deseq2 column headers to match RNA-Seq headers
torres-alexis Jun 25, 2025
27ecec8
Update ancombc column headers to match format of deseq2 headers, upda…
torres-alexis Jun 25, 2025
0826a15
Add pipeline document changes for plot taxonomy, alpha diversity, bet…
torres-alexis Jun 28, 2025
f441284
Add pipelinme documentation changes for AmpIllumina contrasts change
torres-alexis Jul 3, 2025
7587b81
update download.file command
torres-alexis Jul 8, 2025
a780400
Add pipeline documentation update for single contrasts table
torres-alexis Jul 9, 2025
e06d621
Fix typos
torres-alexis Jul 10, 2025
5ad4475
NF-AmpIllumina pipeline document updates associated with GeneLab_Ampl…
asaravia-butler Jul 11, 2025
730193a
Add new r packages to pipeline doc
torres-alexis Jul 28, 2025
d6f55ce
fix typos
torres-alexis Jul 29, 2025
d06ca80
Add software packages and typo fixes
asaravia-butler Aug 5, 2025
d1680cf
Merge branch 'master' into DEV_Amplicon_Illumina_NF_Conversion_original
bnovak32 Aug 14, 2025
5813d48
updated licenses
bnovak32 Aug 14, 2025
5693642
Moved NF_AmpIllumina to its own repository and added back as submodule
bnovak32 Aug 14, 2025
a0023c4
Updated Workflow Doc README
bnovak32 Aug 14, 2025
0b9a9b0
Manually apply updates from dev2-amplicon-add-runsheet-visualizations
bnovak32 Aug 14, 2025
cb25d9f
Merge pull request #171 from bnovak32/DEV_Amplicon_Illumina_NF_Conver…
bnovak32 Aug 14, 2025
450d0da
fixed metagenomics license table links
bnovak32 Aug 14, 2025
d8c7ae1
updated broken links in Amplicon folder
bnovak32 Aug 14, 2025
cce91d5
updated broken license link in Metagenomics README
bnovak32 Aug 14, 2025
d81a184
Fixed typos in Amplicon documents
bnovak32 Aug 14, 2025
84155fc
updated license info in repo main README
bnovak32 Aug 15, 2025
8f495a0
Update GL-DPPD-7104-C.md
bnovak32 Aug 15, 2025
0db1de1
Update OSDR links in README.md
bnovak32 Aug 15, 2025
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3 changes: 3 additions & 0 deletions .gitmodules
Original file line number Diff line number Diff line change
@@ -0,0 +1,3 @@
[submodule "Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina"]
path = Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina
url = https://github.com/nasa/GeneLab_AmpliconSeq_Workflow
37 changes: 0 additions & 37 deletions 3rd_Party_Licenses/Amplicon_and_Metagenomics_3rd_Party_Software.md

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24 changes: 24 additions & 0 deletions 3rd_Party_Licenses/Metagenomics_3rd_Party_Software.md
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## The NASA GeneLab [Metagenomics](../Metagenomics) Processing Pipelines software also makes use of the following 3rd party Open Source software:

|3rd Party Software Name|License|License URL|Copyright Notice|
|:----------------------|:------|:----------|:----------------------|
|FastQC|[GNU GENERAL PUBLIC LICENSE Version 2, June 1991](Metagenomics_3rd_Party_Software_Licenses/FASTQC_LICENSE.pdf)|[https://github.com/s-andrews/FastQC/blob/master/LICENSE](https://github.com/s-andrews/FastQC/blob/master/LICENSE)|Copyright (C) 1989, 1991 Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|MultiQC|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/MultiQC_LICENSE.pdf)|[https://github.com/ewels/MultiQC/blob/master/LICENSE](https://github.com/ewels/MultiQC/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|bbduk|[BBTools Copyright (c) 2014, The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy). All rights reserved.](Metagenomics_3rd_Party_Software_Licenses/bbduk_license.pdf)|[https://github.com/BioInfoTools/BBMap/blob/a9ceda047a7c918dc090de0fdbf6f924292d4a1f/license.txt](https://github.com/BioInfoTools/BBMap/blob/a9ceda047a7c918dc090de0fdbf6f924292d4a1f/license.txt)|BBTools Copyright (c) 2014, The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy). All rights reserved.|
|megahit|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/megahit_LICENSE.pdf)|[https://github.com/voutcn/megahit/blob/master/LICENSE](https://github.com/voutcn/megahit/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|bit|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/bit_LICENSE.pdf)|[https://github.com/AstrobioMike/bioinf_tools/blob/master/LICENSE](https://github.com/AstrobioMike/bioinf_tools/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <https://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|bowtie2|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/bowtie2_LICENSE.pdf)|[https://github.com/BenLangmead/bowtie2/blob/master/LICENSE](https://github.com/BenLangmead/bowtie2/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|samtools|[The MIT/Expat License Copyright (C) 2008-2020 Genome Research Ltd.](Metagenomics_3rd_Party_Software_Licenses/samtools_LICENSE.pdf)|[https://github.com/samtools/samtools/blob/develop/LICENSE](https://github.com/samtools/samtools/blob/develop/LICENSE)|Copyright (C) 2008-2021 Genome Research Ltd. Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|prodigal|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/Prodigal_LICENSE.pdf)|[https://github.com/hyattpd/Prodigal/blob/GoogleImport/LICENSE](https://github.com/hyattpd/Prodigal/blob/GoogleImport/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|KOFamScan|[MIT License Copyright (c) 2019 Takuya Aramaki](Metagenomics_3rd_Party_Software_Licenses/kofam_scan_LICENSE.pdf)|[https://github.com/takaram/kofam_scan/blob/master/LICENSE.txt](https://github.com/takaram/kofam_scan/blob/master/LICENSE.txt)|Copyright (c) 2019 Takuya Aramaki Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|CAT|[The MIT License (MIT) Copyright (c) 2019 Universiteit Utrecht](Metagenomics_3rd_Party_Software_Licenses/CAT_LICENSE.pdf)|[https://github.com/dutilh/CAT/blob/master/LICENSE.md](https://github.com/dutilh/CAT/blob/master/LICENSE.md)|Copyright (c) 2019 Universiteit Utrecht Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|Metabat2|[MetaBAT (2014-075), The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy). All rights reserved.](Metagenomics_3rd_Party_Software_Licenses/MetaBAT_license.pdf)|[https://bitbucket.org/berkeleylab/metabat/src/master/license.txt](https://bitbucket.org/berkeleylab/metabat/src/master/license.txt)|MetaBAT (2014-075), The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy). All rights reserved.|
|checkm|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/CheckM_LICENSE.pdf)|[https://github.com/Ecogenomics/CheckM/blob/master/LICENSE](https://github.com/Ecogenomics/CheckM/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|gtdbtk|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/GTDBTk_LICENSE.pdf)|[https://github.com/Ecogenomics/GTDBTk/blob/master/LICENSE](https://github.com/Ecogenomics/GTDBTk/blob/master/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|KEGGDecoder|[The MIT License (MIT) Copyright (c) 2019 Benjamin Tully](Metagenomics_3rd_Party_Software_Licenses/KEGGDecoder_LICENSE.pdf)|[https://github.com/bjtully/BioData/blob/master/LICENSE](https://github.com/bjtully/BioData/blob/master/LICENSE) | Copyright (c) 2019 Benjamin Tully Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|HUMAnN3|[The HUMAnN software is licensed under the MIT license. Copyright (c) 2014 Harvard School of Public Health](Metagenomics_3rd_Party_Software_Licenses/HUMAnN_LICENSE.pdf)|[https://github.com/biobakery/humann/blob/master/LICENSE](https://github.com/biobakery/humann/blob/master/LICENSE)|Copyright (c) 2014 Harvard School of Public Health Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|MetaPhlAn3|[The MIT License (MIT) Copyright (c) 2015, Duy Tin Truong, Nicola Segata and Curtis Huttenhower](Metagenomics_3rd_Party_Software_Licenses/MetaPhlAn3_license.pdf)|[https://github.com/biobakery/MetaPhlAn/blob/3.0/license.txt](https://github.com/biobakery/MetaPhlAn/blob/3.0/license.txt)|Copyright (c) 2015, Duy Tin Truong, Nicola Segata and Curtis Huttenhower|
|kraken2|[The MIT License (MIT) Copyright (c) 2017-2018 Derrick Wood](Metagenomics_3rd_Party_Software_Licenses/kraken2_LICENSE.pdf)|[https://github.com/DerrickWood/kraken2/blob/master/LICENSE](https://github.com/DerrickWood/kraken2/blob/master/LICENSE)|Copyright (c) 2017-2021 Derrick Wood Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|Snakemake|[The MIT License (MIT) Copyright (c) 2012-2019 Johannes Köster](Metagenomics_3rd_Party_Software_Licenses/License_Snakemake_6.7.0_documentation.pdf)|[https://snakemake.readthedocs.io/en/stable/project_info/license.html](https://snakemake.readthedocs.io/en/stable/project_info/license.html)|Copyright (c) 2012-2019 Johannes Köster <[email protected]> Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:|
|genelab-utils|[GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/genelab-utils_LICENSE.pdf)|[https://github.com/AstrobioMike/GeneLab-utils/blob/main/LICENSE](https://github.com/AstrobioMike/GeneLab-utils/blob/main/LICENSE)|Copyright (C) 2007 Free Software Foundation, Inc <http://fsf.org/> Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
|R|[GNU GENERAL PUBLIC LICENSE Version 2, June 1991, and Version 3, 29 June 2007](Metagenomics_3rd_Party_Software_Licenses/R_GPL-2_and_GPL-3_LICENSES.pdf)|[https://www.r-project.org/Licenses/](https://www.r-project.org/Licenses/)|Version 2: Copyright (C) 1989, 1991 Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.; Version 3: Copyright (C) 2007 Free Software Foundation, Inc. http://fsf.org/ Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.|
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## The NASA GeneLab Processing Pipelines utilize 3rd party Open Source software as indicated:

| Assay Type | 3rd Party Software Table | 3rd Party Software Licenses |
|:-------------------------------|:-------------------------|:----------------------------|
| Amplicon Sequencing | [Amplicon 3rd Party Software](https://github.com/nasa/GeneLab_AmpliconSeq_Workflow/tree/main/License/3rd_Party_Licenses/README.md) | [Amplicon 3rd Party Licenses](https://github.com/nasa/GeneLab_AmpliconSeq_Workflow/tree/main/License/3rd_Party_Licenses) |
| Metagenomics | [Metagenomics 3rd Party Software](./Metagenomics_3rd_Party_Software.md) | [Metagenomics 3rd Party Licenses](./Metagenomics_3rd_Party_Software_Licenses/) |
| Methylation Sequencing | [Methyl-Seq 3rd Party Software](./Methyl-Seq_3rd_Party_Software.md) | [Methyl-Seq 3rd Party Licenses](./Methyl-Seq_3rd_Party_Software_Licenses/) |
| Microarray - Affymetrix | [Microarray Affymetrix 3rd Party Software](./Microarray_Affymetrix_3rd_Party_Software.md) | [Microarray Affymetrix 3rd Party Licenses](./Microarray_Affymetrix_3rd_Party_Software_Licenses/) |
| Microarray - Agilent 1-channel | [Microarray Agilent 1-channel 3rd Party Software](./Microarray_Agilent_1_Channel_3rd_Party_Software.md) | [Microarray Affymetrix 3rd Party Licenses](./Microarray_Agilent_1_Channel_3rd_Party_Software_Licenses/) |
| (bulk) RNAseq | [RNASeq 3rd Party Software](./RNAseq_3rd_Party_Software.md) | [RNAseq 3rd Party Licenses](./RNAseq_3rd_Party_Software_Licenses/) |
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---

<p align="center">
<a href="../images/GL-amplicon-overview.pdf"><img src="../images/GL-amplicon-overview.png"></a>
<a href="../images/GL-amplicon-subwayplot.pdf"><img src="../images/GL-amplicon-subwayplot.png"></a>
</p>

---
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