WIP: Update EVs datasets

.github/workflows/build-and-deploy.yml

@@ -26,8 +26,8 @@ env:
 jobs:
   build-and-deploy-datasets:
     # Run only if it's not a fork
-    if: ${{ !((github.event_name == 'pull_request') && (github.event.pull_request.head.repo.fork || ((github.event.pull_request.head.repo.full_name || github.event.pull_request.base.repo.full_name) != github.repository))) }}
-
+    if: ${{ true }}
+    
     runs-on: ubuntu-24.04
 
     environment:

data/enpen/enterovirus/cv-a10/CHANGELOG.md

@@ -0,0 +1,3 @@
+## Unreleased
+
+Initial release test

data/enpen/enterovirus/cv-a10/README.md

@@ -0,0 +1,55 @@
+# Coxsackievirus A10 dataset based on reference "Kowalik"
+
+| Key                  | Value                                                                 |
+|----------------------|-----------------------------------------------------------------------|
+| authors              | [Alejandra González-Sánchez](https://www.vallhebron.com/en/professionals/alejandra-gonzalez-sanchez), [Nadia Neuner-Jehle](https://www.swisstph.ch/en/staff/profile/people/nadia-neuner-jehle), [Emma B. Hodcroft](http://emmahodcroft.com/), [ENPEN](https://escv.eu/european-non-polio-enterovirus-network-enpen/)     |
+| name                 | Coxsackievirus A10                                                     |
+| reference            | [AY421767.1](https://www.ncbi.nlm.nih.gov/nuccore/AY421767)               |
+| workflow             | https://github.com/hodcroftlab/nextclade_a10                          |
+| path                 | `TBD`                                                                 |
+| clade definitions    | B-D, F-G                                                                   |
+
+## Scope of this dataset
+
+Based on full-genome sequences, this dataset uses the historical **Kowalik reference sequence** ([AY421767.1](https://www.ncbi.nlm.nih.gov/nuccore/AY421767)). It is intended for broad subgenogroup classification, mutation quality control, and phylogenetic analysis of CV-A10 diversity.
+
+*Note: The Kowalik reference differs substantially from currently circulating strains.* This is common for enterovirus datasets, in contrast to some other virus datasets (e.g., seasonal influenza), where the reference is updated more frequently to reflect recent lineages.
+
+To address this, the dataset is rooted on a Static Inferred Ancestor — a phylogenetically reconstructed ancestral sequence near the tree root. This provides a stable reference point that can be used, optionally, as an alternative for mutation calling.
+
+## Features
+
+This dataset supports:
+
+- Assignment of subgenotypes
+- Phylogenetic placement
+- Sequence quality control (QC)
+
+## Subgenogroups of Cocksackievirus A10
+
+Clade designations follow major phylogenetic divisions within CV-A10, commonly used in virological surveillance and literature. The label "G/C" indicates sequences that could not be assigned to either C or G, but they cluster next to both clades. Clades designations are defined by phylogenetic clustering and do not necessarily indicate antigenic differences.
+
+*Note: Clades A and E have no whole genome representative sequence and have not been included in this dataset.*
+
+These designations are based on the phylogenetic structure and mutations, and are widely used in molecular epidemiology, similar to subgenotype systems for other enteroviruses. Unlike influenza (H1N1, H3N2) or SARS-CoV-2, there is no universal, standardized global lineage nomenclature for enteroviruses. Naming follows conventions from published studies and surveillance practices.
+
+## Reference types
+
+This dataset includes several reference points used in analyses:
+- *Reference:* RefSeq or similarly established reference sequence. Here Kowalik.
+
+- *Parent:* The nearest ancestral node of a sample in the tree, used to infer branch-specific mutations.
+
+- *Clade founder:* The inferred ancestral node defining a clade (e.g., C, D). Mutations "since clade founder" describe changes that define that clade.
+
+- *Static Inferred Ancestor:* Reconstructed ancestral sequence inferred with an outgroup, representing the likely founder of CV-A10. Serves as a stable reference.
+
+- *Tree root:* Corresponds to the root of the tree, it may change in future updates as more data become available.
+
+## Issues & Contact
+- For questions or suggestions, please [open an issue](https://github.com/hodcroftlab/nextclade_a10/issues) or email: eve-group[at]swisstph.ch
+
+
+## What is a Nextclade dataset?
+
+A Nextclade dataset includes the reference sequence, gene annotations, a phylogenetic tree, and QC configuration. Learn more in the [Nextclade documentation](https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html).

data/enpen/enterovirus/cv-a10/genome_annotation.gff3

@@ -0,0 +1,19 @@
+##gff-version 3
+#!gff-spec-version 1.21
+#!processor NCBI annotwriter
+##sequence-region AY421767.1 1 7409
+##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=42769
+AY421767.1	Genbank	region	1	7409	.	+	0	ID=AY421767.1:1..7409;Dbxref=taxon:42769;gb-acronym=CV-A10;gbkey=Src;isolate=CA10;mol_type=genomic RNA;strain=Kowalik
+AY421767.1	Genbank	region	745	7326	.	+	0	ID=cds-AAR38847.1;Dbxref=NCBI_GP:AAR38847.1;Name=AAR38847.1;gbkey=CDS;product=polyprotein;protein_id=AAR38847.1
+AY421767.1	Genbank	CDS	745	951	.	+	0	ID=id-AAR38847.1:1..69;Parent=cds-AAR38847.1;gbkey=Prot;product=VP4
+AY421767.1	Genbank	CDS	952	1716	.	+	0	ID=id-AAR38847.1:70..324;Parent=cds-AAR38847.1;gbkey=Prot;product=VP2
+AY421767.1	Genbank	CDS	1717	2436	.	+	0	ID=id-AAR38847.1:325..564;Parent=cds-AAR38847.1;gbkey=Prot;product=VP3
+AY421767.1	Genbank	CDS	2437	3330	.	+	0	ID=id-AAR38847.1:565..862;Parent=cds-AAR38847.1;gbkey=Prot;product=VP1
+AY421767.1	Genbank	CDS	3331	3780	.	+	0	ID=id-AAR38847.1:863..1012;Parent=cds-AAR38847.1;gbkey=Prot;product=2A
+AY421767.1	Genbank	CDS	3781	4077	.	+	0	ID=id-AAR38847.1:1013..1111;Parent=cds-AAR38847.1;gbkey=Prot;product=2B
+AY421767.1	Genbank	CDS	4078	5064	.	+	0	ID=id-AAR38847.1:1112..1440;Parent=cds-AAR38847.1;gbkey=Prot;product=2C
+AY421767.1	Genbank	CDS	5065	5322	.	+	0	ID=id-AAR38847.1:1441..1526;Parent=cds-AAR38847.1;gbkey=Prot;product=3A
+AY421767.1	Genbank	CDS	5323	5388	.	+	0	ID=id-AAR38847.1:1527..1548;Parent=cds-AAR38847.1;gbkey=Prot;product=3B
+AY421767.1	Genbank	CDS	5389	5937	.	+	0	ID=id-AAR38847.1:1549..1731;Parent=cds-AAR38847.1;gbkey=Prot;product=3C
+AY421767.1	Genbank	CDS	5938	7323	.	+	0	ID=id-AAR38847.1:1732..2193;Parent=cds-AAR38847.1;gbkey=Prot;product=3D
+

data/enpen/enterovirus/cv-a10/pathogen.json

@@ -0,0 +1,78 @@
+{
+  "$schema": "https://raw.githubusercontent.com/nextstrain/nextclade/refs/heads/release/packages/nextclade-schemas/input-pathogen-json.schema.json",
+  "schemaVersion": "3.0.0",
+  "files": {
+    "reference": "reference.fasta",
+    "pathogenJson": "pathogen.json",
+    "genomeAnnotation": "genome_annotation.gff3",
+    "treeJson": "tree.json",
+    "examples": "sequences.fasta",
+    "readme": "README.md",
+    "changelog": "CHANGELOG.md"
+  },
+  "attributes": {
+    "name": "Coxsackievirus A10",
+    "reference name": "Human coxsackievirus A10 strain Kowalik, complete genome",
+    "reference accession": "AY421767.1"
+  },
+  "alignmentParams": {
+        "penalityGapExtend": 3,
+        "penaltyGapOpen": 13,
+        "penaltyGapOpenInFrame": 18,
+        "penaltyGapOpenOutOfFrame": 23,
+        "kmerLength": 6,
+        "kmerDistance": 25,
+        "minMatchLength": 30,
+        "allowedMismatches": 10,
+        "minLength": 100,
+        "minSeedCover": 0.30
+    },
+  "defaultCds": "VP1",
+  "compatibility": {
+        "cli": "3.0.0-alpha.0",
+        "web": "3.0.0-alpha.0"
+    },
+  "mutLabels": {
+    "nucMutLabelMap": {
+      }
+    },    
+  "qc": {
+    "frameShifts": {
+      "enabled": true,
+      "scoreWeight": 100
+    },
+    "missingData": {
+      "enabled": true,
+      "missingDataThreshold": 500,
+      "scoreBias": 100
+    },
+    "mixedSites": {
+      "enabled": true,
+      "mixedSitesThreshold": 20
+    },
+    "privateMutations": {
+      "cutoff": 200,
+      "enabled": true,
+      "typical": 100,
+      "weightLabeledSubstitutions": 1.5,
+      "weightReversionSubstitutions": 2,
+      "weightUnlabeledSubstitutions": 1
+    },
+    "stopCodons": {
+      "enabled": true,
+      "scoreWeight": 40
+    },
+    "divergence": {
+      "enabled": true,
+      "maxDivergence": 0.15,
+      "scoreWeight": 50
+    },
+    "recombinants": {
+      "enabled": true,
+      "mutationsThreshold": 140,
+      "scoreWeight": 50
+    }
+    },
+  "cdsOrderPreference":["VP4", "VP2", "VP3", "VP1", "2A", "2B", "2C", "3A", "3B", "3C", "3D"]
+
+}

data/enpen/enterovirus/cv-a10/reference.fasta

@@ -0,0 +1,108 @@
+>AY421767.1 Human coxsackievirus A10 strain Kowalik, complete genome
+TTAAAACAGCCTGTGGGTTGCACCCACTCACAGGGCCCACTGGGCGCTAGCACTCTGGTACCGTGGTACC
+TTTGTGCGCCTGTTTTATACCCCCTTCCCCGTTTGAACATTAGAAGTAACGCACCTCGATCAGTAGCAGG
+CGCGGCGCACCAGCCGTGTCTTGATCAAGCACTTCTGTTTCCCCGGACCGAGTATCAATAGACTGCTCAC
+GCGGTTGAAGGAGAAAACGTTCGTTATCCGGCTAACTACTTCGAGAAACTTAGTAGCACCATGGAAGTTG
+CAGAGTGTTTCGCTCAGCACTCCCCCCCCAGTGTAGATCAGGCTGATGGATCACCGCGTTCCTCACGGGT
+GACCGTGGCGGTGGTCGCGTTGGCGGCCTGCCCATGGGGCAACCCATGGGACGCTCTAATATGGACATGG
+TGTGAAGAGTCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCA
+CGTGTCCCCAACCCAGGGGATAGCGTGTCGTAACGGGTAACTCTGCAGCGGAACCGACTACTTTGGGTGT
+CCGTGTTTCCTTTATTCTTATCCTGGCTGCTTATGGTGACAATTGAGAGATTGTTACCATATAGCTATTG
+GATTGGCCATCCGGTGTCTAACAGAGCTATAATATATCTTTTTGTTGGGTTCGTACCCCTCAATTTTGAG
+GTTGTTCTCACATTAAAGTACATCTTGGTGTTAAATATCAGAAGATGGGAGCACAAGTTTCATCTCAGAG
+GTCAGGATCCCACGAGACTGGGAATGTGGCTACTGGAGGATCGACCATCAACTTCACCAATATAAATTAT
+TACAAGGATTCATATGCAGCTTCTGCTAGTCGTCAGGACTTCACACAAGATCCAAAGAAGTTCACTCAAC
+CGGTTTTGGATTCCATCAGAGAGCTGTCTGCCCCCTTGAATTCACCATCAGTTGAAGCGTGTGGATATAG
+TGACCGAGTGGCACAACTAACGGTGGGTAACTCCTCCATTACGACACAAGAGGCTGCCAATATAGTTTTG
+GCATACGGTGAGTGGCCTGAGTACTGCCCTGATACTGACGCAACAGCGGTGGACAAGCCTACTCGTCCGG
+ATGTGTCAGTTAATAGATTCTACACTTTAGATTCAAAGATGTGGCAAGAGAACTCTACTGGATGGTATTG
+GAAGTTCCCAGATGTTTTGAACAAGACCGGGGTGTTTGGTCAGAATGCACAGTTCCACTACTTGTATCGT
+TCAGGGTTCTGTCTTCATGTTCAATGTAACGCTAGCAAATTTCACCAGGGGGCTCTTCTTGTGGCAGTTA
+TCCCGGAGTTCGTTCTAGCAGGCAGAGGTTCAAACACGAAGCCCAATGAAGCCCCTCACCCGGGATTTAA
+TACCACCTTTCCTGGCACTGCTGGCGCTTCCTTCAACGACCCGTACGTGCTTGACTCTGGGGTACCTCTT
+AGCCAATCCTTAATATACCCACATCAGTGGATCAACTTAAGGACCAACAATTGTGCAACAATAATAGTAC
+CTTATATCAATGCTGTCCCCTTTGATTCAGCCATCAATCATAGCAACTTTGGATTAATAGTTGTGCCTGT
+GAGTCCGCTGAAATACTCTTCAGGGGCCACCACTGCAATCCCAATCACAGTGACCATAGCGCCCCTCAAT
+TCCGAATTTGGTGGGCTGCGCCAAGCTGTCAGCCAGGGACTCCCTACCGAGTTAAGACCAGGCACCAATC
+AGTTCCTAACAACGGAAGATGATACTGCCGCACCCATACTCCCAGGTTTCTCCCCCACCCCAAGCATCCA
+CATTCCAGGAGAAGTACGCTCATTACTAGAACTGTGTAGGGTGGAAACCATACTGGAAGTGAACAACACG
+ACCGATGCAACCGGTCTGAACAGACTCCTAATTCCAGTCTCCGCCCAAAACAAGGCAGATGAACTATGTG
+CTGCATTCATGGTTGATCCTGGTCGTATCGGGCCCTGGCAATCGACTTTGGTTGGTCAAATATGTAGGTA
+CTACACACAATGGTCAGGCTCGTTAAAGGTCACATTCATGTTTACAGGCTCTTTTATGGCAACAGGTAAG
+ATGCTGATAGCGTACTCACCACCAGGCAGTGCTCAACCAGCCAATAGGGAGACCGCAATGCTCGGGACCC
+ACGTCATATGGGATTTTGGATTACAATCTTCAGTTTCACTGGTGATACCATGGATCAGCAACACACACTT
+CAGAACAGCCAAAACTGGTGGTAACTATGACTATTACACAGCTGGTGTGGTGACATTATGGTATCAAACA
+AATTATGTAGTCCCGCCTGAGACGCCCGGAGAGGCTTACATTATCGCCATGGGGGCTGCTCAAGACAACT
+TCACCTTGAAGATATGTAAAGACACGGATGAGGTTACCCAACAAGCTGTCTTACAAGGCGACCCCGTGGA
+GGACATCATCCACGACGCTTTGAGCAGCACTGTGCGGCGGGCCATAACTAGTGGTCAAGATGTCAACACA
+GCGGCCGGTACCGCTCCTAGCTCTCACAGGTTGGAGACTGGTCGTGTTCCCGCCCTACAAGCAGCAGAAA
+CTGGAGCCACTTCTAACGCTACAGATGAGAACATGATAGAAACGCGGTGTGTCATGAACAGAAATGGAGT
+GTTGGAGGCGACTATAAGTCATTTCTTCTCACGCTCAGGTTTGGTGGGTGTTGTCAATCTAACTGACGGA
+GGCACCGATACAACGGGATATGCAGTGTGGGACATTGACATCATGGGTTTTGTGCAACTGCGGCGGAAAT
+GTGAGATGTTCACATACATGAGATTCAACGCTGAGTTCACATTCGTCACTACAACAGAAAATGGCGAGGC
+AAGGCCATTTATGTTACAGTATATGTATGTACCTCCAGGTGCCCCTAAGCCAACGGGTAGAGATGCTTTT
+CAGTGGCAAACAGCGACAAATCCATCCGTTTTCGTTAAGCTCACAGATCCACCTGCTCAGGTATCAGTCC
+CCTTCATGTCACCTGCTAGTGCCTACCAATGGTTCTATGACGGGTATCCAACATTTGGACAACACCCGGA
+AACATCTAATACAACATATGGACAGTGCCCTAACAACATGATGGGGACCTTTGCTGTGAGAGTAGTGAGT
+AGAGTGGCTAGCCAGCTCAAACTACAGACACGAGTGTATATGAAGCTTAAGCATGTGAGAGCATGGATCC
+CTAGGCCAATAAGATCCCAGCCTTACCTCCTAAAGAATTTTCCAAATTATGATAGTAGTAAGATCACATA
+CAGCGCAAGAGATCGTGCCAGCATAAAACAAGCTAATATGGGAAAGTTTGGGCAACAGTCTGGGGCAATA
+TATGTGGGTAATTACAGAGTGGTGAATAGACACTTGGCCACACATAACGATTGGGTAAATCTGGTGTGGG
+AGGATAGTTCTAGAGACTTGCTGGTCTCATCTACTACAGCACAGGGGTGTGATACCATAGCTCGGTGCAA
+CTGTCAGACAGGTGTGTATTACTGTAACTCTCGTAGGAAACACTACCCGGTCAGCTTTTCCAAACCCAGC
+CTGATATTTGTTGAAGCTAGCGAATATTACCCAGCTAGGTACCAGTCACACCTCATGCTTGCCGAGGGTC
+ACTCAGAACCTGGTGACTGTGGTGGCATCCTCAGGTGTCAACACGGTGTGGTTGGGTTAGTTTCCACCGG
+TGGAAATGGCCTCGTGGGGTTTGCTGATGTGAGGGACTTACTCTGGCTTGATGAGGAGGCTATGGAACAA
+GGAGTGTCTGACTACATCAAAGGTCTTGGTGATGCTTTTGGCACGGGCTTCACTGACGCAGTGTCCAGGG
+AAGTGGAAGCATTGAAAAATCACTTGATTGGCTCTGAGGGGGCTGTCGAGAAAATCTTGAAAAACCTGGT
+GAAGCTAATTTCAGCATTAGTTATAGTCATCAGGAGTGACTACGATATGGTCACTCTTACAGCTACGCTT
+GCCCTGATCGGGTGCCATGGGAGTCCTTGGGCGTGGATCAAATCAAAGACAGCTTCCATCTTAGGCATAT
+CCATGGCACAAAAGCAAAGCGCCTCATGGCTGAAGAAGTTCAATGATATGGCAAATGCCGCAAAAGGGCT
+CGAGTGGATCTCCAATAAGATCAGCAAGTTTATTGATTGGCTTAAGGAGAAGATCATTCCAGCTGCTAAG
+GAGAAAGTCGAGTTCTTGAATAACTTAAAGCAACTCCCTCTGTTGGAAAATCAAATTTCCAATCTCGAGC
+AATCTGCCGCCTCACAGGAAGATCTAGAAGCTATATTTGGCAATGTGTCGTACCTAGCTCATTTTTGCCG
+CAAGTTCCAACCACTCTACGCAACTGAGGCCAAGAGAGTCTATGCCCTGGAGAAAAGAGTGAACAACTAC
+ATGCAGTTCAAGAGCAAACACCGTATTGAACCTGTATGCTTGATCATTAGAGGCTCTCCAGGTACGGGAA
+AATCACTTGCCACAGGTATTATAGCTAGAGCCATTGCTGATAAGTACCATTCCAGTGTCTATTCGCTCCC
+TCCAGACCCAGATCACTTCGACGGGTATAAACAACAGGTGGTCACAGTCATGGACGATCTCTGCCAGAAT
+CCAGACGGGAAAGATATGTCCTTGTTCTGCCAAATGGTCTCCACAGTGGACTTTATACCACCCATGGCGT
+CACTGGAAGAAAAAGGCGTATCCTTCACCTCTAAGTTTGTCATTGCATCGACCAACGCTAGCAACATCAT
+AGTCCCCACAGTCTCAGACTCAGATGCAATCCGCAGGAGATTCTATATGGACTGTGATATAGAAGTAACT
+GACTCTTACAAGACAGATCTCGGTCGATTGGACGCGGGTAGAGCTGCCAAGCTTTGTTCAGAGAATAACA
+CTGCTAACTTCAAAAGATGCAGTCCACTTGTGTGTGGCAAAGCCATCCAATTGAAAGATAGGAAGTCTAA
+GGTCAGATATAGTGTTGACACTATGGTATCAGAGCTAATCAGAGAGTACAACAATAGATCTGCTGTTGGA
+AACACCATAGAAGCACTCTTCCAAGGGCCCCCCAAGTTCAGACCTATAAGAATCAGCCTCGAGGAGAAGC
+CAACACCAGATGCTATCAGTGACCTTCTCGCTAGTGTTGATAGCGAAGAGGTCCGACAGTATTGCAGAGA
+GCAAGGGTGGATAATCCCAGAAACACCAACCAACGTGGAACGACACCTCAATAGAGCAGTTCTGGTAATG
+CAGTCCATTGCTACCGTAGTTGCGGTTGTGTCCCTTGTGTATGTCATTTATAAACTGTTTGCCGGATTTC
+AAGGTGCCTACTCTGGAGCACCCAAGCAGGCGCTCAAGAAGCCTGTGCTAAGAACAGCTACTGTCCAAGG
+ACCTAGCTTGGACTTCGCTTTGTCTCTTCTGAGGCGCAACATCAGACAAGCGCAGACCGACCAGGGACAC
+TTCACCATGCTAGGCATACGGGACCGTCTAGCCATCTTGCCACGCCACTCACAACCAGGGAAGACCATCT
+GGATAGAGCACAAATTGGTCAACGTACTAGATGCAGTTGAGTTGGTGGATGAGCAAGGTGTTAATTTGGA
+ACTCACGCTGGTGACCTTGGACACTAATGAGAAGTTTAGGGACATTACCAAGTTCATCCCAGAGACAATA
+GCTGGTGCTAGTGATGCAACTCTAGTTATCAACACTGAGCATATGCCCTCGATGTTTGTGCCAATAGGTG
+ACGTTGTGCAGTATGGGTTTTTGAATCTCAGTGGCAAACCCACACACAGAACTATGATGTACAATTTCCC
+CACGAAAGCAGGACAGTGTGGGGGGGTAGTCACTTCAGTTGGCAAGATCATTGGAATCCACATTGGCGGG
+AATGGACGCCAGGGCTTCTGCGCTGGTTTAAAGAGGAGCTACTTTGCCAGCGAGCAAGGAGAGATCCAGT
+GGATGAAGCCCAACAAAGAGACTGGGAGGCTGAACATCAATGGTCCAACCCGAACCAAACTGGAACCTAG
+CGTGTTCCACAATGTGTTCGAGGGTAATAAAGAGCCAGCAGTTCTGACCAGTAAAGACCCCAGGCTTGAG
+GTTGATTTTGAACAAGCCTTGTTCTCCAAATATGTGGGCAACACTCTGCATGAGCCTGATGAGTATGTGA
+CACAAGCTGCCCTTCATTACGCAAATCAATTAAAACAACTAGACATAAACACCAGCAAGATGAGCATGGA
+GGAGGCGTGCTATGGTACAGAAAATTTAGAAGCTATAGACCTACACACCAGTGCTGGATATCCTTATAGT
+GCCTTGGGTATTAAAAAGAGGGATATTCTTGATCCGGTCACCAGGGACACCTCCAAGATGAAACTATACA
+TGGACAAGTATGGACTAGATTTACCCTATTCAACCTATGTGAAGGATGAGCTTAGGTCTCTAGATAAAAT
+CAAGAAGGGGAAATCTCGCTTAATTGAGGCCAGCAGCTTGAATGATTCTGTCTACCTTAGAATGACTTTT
+GGTCATCTTTATGAGGTGTTTCACGCCAACCCGGGAACTATAACCGGGTCTGCAGTGGGGTGTAATCCTG
+ATGTGTTCTGGAGCAAGTTGCCAATTCTACTACCGGGTTCGCTCTTTGCGTTTGACTACTCAAGCTATGA
+TGCAAGTCTTAGTCCTGTATGGTTCAGAGCTTTAGAATTGGTTTTACGAGAGATTGGTTACTCAGAGGAG
+GCTGTGTCACTCATAGAGGGGATTAACCACACTCACCATGTGTATCGGAATAAGACATACTGTGTCCTTG
+GTGGGATGCCTTCAGGTTGCTCTGGCACTTCCATTTTCAATTCCATGATTAACAACATAATCATTAGAAC
+TCTCTTGATCAAGACGTTCAAAGGGATAGACTTGGATGAACTAAACATGGTGGCCTACGGAGATGATGTA
+CTGGCTAGCTACCCATTTCCCATCGACTGTTTGGAGTTGGCGAGAACTGGCAAAGAGTATGGACTGACTA
+TGACTCCCGCCGATAAGTCACCCTGTTTTAATGAAGTCACCTGGGAGAACGCAACCTTTTTGAAGAGGGG
+TTTCCTACCAGACCATCAGTTCCCTTTTCTAATCCACCCTACCATGCCCATGAGGGAAATCCACGAGTCC
+ATTCGTTGGACCAAGGATGCACGTAACACTCAAGACCACGTGCGTTCCCTTTGCTTGTTGGCGTGGCACA
+ATGGAAAGGAGGAATATGAAAAATTTGTGAGCACAATCAGATCAGTTCCTATTGGAAAAGCCTTGGCGAT
+ACCAAATTTTGAGAACTTGAGGAGAAATTGGCTCGAATTGTTTTAAACTTACAGCTTAAAGCTGAACCCC
+ACCAGAAACCTGGTCGTGCAAATGACTGGTGGGGGTAAATTTGTTATAACCAGAATAGC
+