plastid metagene_generate, make_wiggle, psite

modules/nf-core/plastid/make_wiggle/environment.yml

@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::plastid=0.6.1

modules/nf-core/plastid/make_wiggle/main.nf

@@ -0,0 +1,64 @@
+process PLASTID_MAKE_WIGGLE {
+    tag "$meta.id"
+    label "process_single"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/plastid:0.6.1--py39had3e4b6_2':
+        'biocontainers/plastid:0.6.1--py39had3e4b6_2' }"
+
+    input:
+    tuple val(meta), path(bam), path(bam_index), path(p_offsets)
+    val min_length
+    val output_format
+    val fiveprime_variable
+
+    output:
+    tuple val(meta), path("*.{wig,bedgraph}"), emit: tracks
+    path "versions.yml", emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = output_format == "bedgraph" ? "bedgraph" : "wig"
+    def args = task.ext.args ?: ""
+    if (fiveprime_variable) args += "--fiveprime_variable"
+    """
+    make_wiggle \\
+        --count_files "$bam" \\
+        --offset "$p_offsets" \\
+        --min_length "$min_length" \\
+        --output_format "$output_format" \\
+        --verbose \\
+        -o "$prefix" \\
+        $args
+
+    sed -i '/^##/d' *.wig # remove variable comment header
+
+    if [ "$output_format" = "bedgraph" ]; then
+        for FILE in *.wig; do
+            mv "\$FILE" "\${FILE%.wig}.bedgraph"
+        done
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plastid: 0.6.1
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = output_format == "bedgraph" ? "bedgraph" : "wig"
+    """
+    touch ${prefix}_fw.$extension
+    touch ${prefix}_rc.$extension
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plastid: 0.6.1
+    END_VERSIONS
+    """
+}

modules/nf-core/plastid/make_wiggle/meta.yml

@@ -0,0 +1,80 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "plastid_make_wiggle"
+description: Create wiggle or bedGraph files from alignment files after
+  applying a read mapping rule (e.g. to map ribosome-protected footprints at
+  their P-sites), for visualization in a genome browser
+keywords:
+  - genomics
+  - riboseq
+  - psite
+  - wiggle
+  - bedgraph
+tools:
+  - "plastid":
+      description: "Nucleotide-resolution analysis of next-generation sequencing and genomics data"
+      homepage: "https://github.com/joshuagryphon/plastid"
+      documentation: "https://plastid.readthedocs.io/en/latest/"
+      tool_dev_url: "https://github.com/joshuagryphon/plastid"
+      doi: "http://dx.doi.org/10.1186/s12864-016-3278-x"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:plastid
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - bam:
+        type: file
+        description: Genome BAM file
+        pattern: "*.bam"
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+    - bam_index:
+        type: file
+        description: Genome BAM index file
+        pattern: "*.bai"
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # BAI
+    - p_offsets:
+        type: file
+        description: Selected p-site offset for each read length (output from plastid_psites)
+        pattern: "*_p_offsets.txt"
+        ontologies: []
+  - min_length:
+      type: integer
+      description: Minimum length of reads to consider
+  - output_format:
+      type: string
+      description: `variable_step` or `bedgraph`
+  - fiveprime_variable:
+      type: boolean
+      description: |
+        Map read alignment to a variable offset from 5' position of read, with
+        offset determined by read length
+output:
+  tracks:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.${extension}":
+          type: file
+          description: wig/bedgraph tracks for forward and reverse strands
+          pattern: "*.${extension}"
+          ontologies:
+            - edam: http://edamontology.org/format_3005 # wig
+            - edam: http://edamontology.org/format_3583 # bedgraph
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@suhrig"
+maintainers:
+  - "@suhrig"

modules/nf-core/plastid/make_wiggle/tests/main.nf.test

@@ -0,0 +1,69 @@
+// nf-core modules test plastid
+nextflow_process {
+
+    name "Test Process PLASTID_MAKE_WIGGLE"
+    script "../main.nf"
+    process "PLASTID_MAKE_WIGGLE"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "plastid"
+    tag "plastid/make_wiggle"
+
+    test("human chr20 bam") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'SRX11780887' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/aligned_reads/SRX11780887_chr20.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/aligned_reads/SRX11780887_chr20.bam.bai", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/plastid/SRX11780887_p_offsets.txt", checkIfExists: true)
+                    ]
+                input[1] = 23 // min_length
+                input[2] = 'variable_step' // output_format
+                input[3] = true // fiveprime_variable
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.tracks).match('wig') },
+                { assert snapshot(process.out.versions).match('versions') }
+            )
+        }
+    }
+
+    test("human chr20 bam -stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'SRX11780887' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/aligned_reads/SRX11780887_chr20.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/aligned_reads/SRX11780887_chr20.bam.bai", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/riboseq_expression/plastid/SRX11780887_p_offsets.txt", checkIfExists: true)
+                    ]
+                input[1] = 23 // min_length
+                input[2] = 'variable_step' // output_format
+                input[3] = true // fiveprime_variable
+                """
+           }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.tracks).match('wig_stub') },
+                { assert snapshot(process.out.versions).match('versions_stub') }
+            )
+        }
+    }
+
+}

modules/nf-core/plastid/make_wiggle/tests/main.nf.test.snap

@@ -0,0 +1,66 @@
+{
+    "versions_stub": {
+        "content": [
+            [
+                "versions.yml:md5,4ecd455575ab11a0ad86dc5e0a6c6959"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.3",
+            "nextflow": "25.10.0"
+        },
+        "timestamp": "2025-12-04T21:33:55.015767252"
+    },
+    "wig": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "SRX11780887"
+                    },
+                    [
+                        "SRX11780887_fw.wig:md5,647f8b1b990e3a7f13f761bad68646fe",
+                        "SRX11780887_rc.wig:md5,17955523d22a9d9bc67a39228cdda343"
+                    ]
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.3",
+            "nextflow": "25.10.0"
+        },
+        "timestamp": "2025-12-04T21:33:49.241128201"
+    },
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ecd455575ab11a0ad86dc5e0a6c6959"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.3",
+            "nextflow": "25.10.0"
+        },
+        "timestamp": "2025-12-04T21:33:49.263313286"
+    },
+    "wig_stub": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "SRX11780887"
+                    },
+                    [
+                        "SRX11780887_fw.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "SRX11780887_rc.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.3",
+            "nextflow": "25.10.0"
+        },
+        "timestamp": "2025-12-04T21:33:55.001327109"
+    }
+}

modules/nf-core/plastid/metagene_generate/environment.yml

@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::plastid=0.6.1

modules/nf-core/plastid/metagene_generate/main.nf

@@ -0,0 +1,44 @@
+process PLASTID_METAGENE_GENERATE {
+    tag "$gtf"
+    label "process_low"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/plastid:0.6.1--py39had3e4b6_2':
+        'biocontainers/plastid:0.6.1--py39had3e4b6_2' }"
+
+    input:
+    path gtf
+    val landmark
+
+    output:
+    path "*_rois.txt"  , emit: rois_txt
+    path "*_rois.bed"  , emit: rois_bed
+    path "versions.yml", emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    metagene generate "${gtf.baseName}" --landmark "$landmark" --annotation_files "$gtf" $args
+    sed -i '/^##/d' *_rois.* # remove variable comment header
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plastid: 0.6.1
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch ${gtf.baseName}_rois.txt
+    touch ${gtf.baseName}_rois.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plastid: 0.6.1
+    END_VERSIONS
+    """
+}