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🧬Rachel's Bioinformatics Pipelines

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⚠️A word of warning...

This repository contains 'omics data-type based pipelines from my general role as a bioinformatician. These are not cleaned, nor are they transportable. However, I figured they are worth allowing others to use if they want!

▶️ Usage

  • To run many of these scripts, users must have access to a high performance computing environment. They are often in pairs with the same name, with the shell script (ex. shell.sh) created to submit the working script (shell.R) to an HPC environment.
  • The user must update the bash script to the appropriate parameters for their unique HPC. These were built on an HPC using command line with SLURM.

🚀Current pipelines

Pipeline Description
🧬 Bulk RNAseq Basic pipeline for bulk RNA-sequencing experiments from preprocessing to downstream pathway analysis
📦 PackageBuilding Helper scripts for building an R package
📊 Visualizations Basic visualization scripts for common plots such as volcano plots
🎯 miRNA Scripts for analysis of miRNA
🧬 scDNAseq/MissionBio Tapestri Analysis scripts for MissionBio Tapestri platform data using the optima package and hardcoding analysis
🔍 scRNAseq Basic analysis for scRNAseq from 10X platforms
🧭 spatial RNAseq/VisiumHD Scripts from analyses of Visium HD samples for preprocessing, quality control, integration using scVI, nucleus segmentation using stardist, differential expression analysis, cell type ID via RCTD

🗓️ Last Updated

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✍️ Maintained by Rachel Griffard-Smith
📫 Contact: Leave a note in the Issues or Pull requests section

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Collections of scripts and pipelines for bioinformatics data

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