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@JannesSP JannesSP commented Sep 8, 2025

  • I do not know anymore in which version, but in some 0.9.x dorado version, the shift and scale values were taken from the raw DACS values instead of the pA signal
  • First I thought this was a change between the old r9 pore to the new r10 and rna004 flowcells, but I noticed, that the sm and sd values in the basecalled bam changed back to normalize the pA signal. Now I just check if the shift is above 400, then the raw DACS signal is taken for normalization, otherwise the pA signal is taken.

JannesSP added 16 commits April 30, 2025 09:22
- I do not know anymore in which version, but in some 0.9.x dorado version, the shift and scale values were taken from the raw DACS values instead of the pA signal
- First I thought this was a change between the old r9 pore to the new r10 and rna004 flowcells, but I noticed, that the sm and sd values in the basecalled bam changed back to normalize the pA signal. Now I just check if the shift is above 400, then the raw DACS signal is taken for normalization, otherwise the pA signal is taken.
@JannesSP JannesSP merged commit 7935303 into main Sep 8, 2025
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