MS1 Isotope Abundances

[mtaylor0199]

Hi,

Is there a way to export the MS1 isotope abundances? We’re particularly interested in the precursor M+0 to precursor M+1 ratio per peptide in the context of deuterium oxide labeling for determination of protein turnover rates.

Thanks!

Michael

[vdemichev]

Hi Michael,

I think it's not really possible to export. The workaround at the moment would be to use DIA-NN's IDs in Skyline and have it extract & quantify chromatograms.

Best,
Vadim

[mtaylor0199]

Thanks for the reply! That is what we've been doing, but we've been getting bottlenecked by single-threaded functions in Skyline (specifically importing the peak boundaries called by DIA-NN) and were also wondering if the deconvolution algorithms in DIA-NN could get us more reliable isotope ratios for messy peptides

-Michael

[vdemichev]

Cannot promise a timeline, but it's a fairly simple addition, I will add it to the TODO list.