DIA-NN exited directed after starting the fasta library generation

[TomsonChang-MS]

Hello,

I set up a DIA-NN 2.3.2 academic version on an internal NAS server for running larger-scale searches in the future. Unfortunately, when we first generated the predicted library from a given human fasta file, it directly exited without any output.

Here's is the log and the settings:

diann.exe --lib "" --threads 40 --verbose 3 --out "D:\DIANN\Predicted_Library\report.parquet" --qvalue 0.01 --matrices --out-lib "D:\DIANN\Predicted_Library\250225_Human_Test.parquet" --gen-spec-lib --predictor --fasta "D:\DIANN\Fasta\250225_Homo_sapiens.fasta" --fasta-search --met-excision --min-pep-len 7 --max-pep-len 30 --min-pr-mz 300 --max-pr-mz 1800 --min-pr-charge 1 --max-pr-charge 4 --min-fr-mz 200 --max-fr-mz 1800 --cut K*,R* --missed-cleavages 1 --unimod4 --rt-profiling

DIA-NN exited

We have checked that the RAM is totally sufficient (1TB) for running a library prediction and reinstalled the DIA-NN, but the problem isn't solved.

[vdemichev]

Hi,

Please try running diann.exe from command line and share what it prints or shows.

Best,
Vadim

[TomsonChang-MS]

Hello Vadim,

Thanks for your quick reply. We will try this ASAP.
Besides, I was just told that this Windows-based NAS server was established recently, and probably no Visual Studio is installed. Is this possible to be a reason for immediate exit?

Best
Tomson

[TomsonChang-MS]

Hello Vadim,

We solved the problem by installing Visual Studio, and now it runs successfully. Thanks for your help.

Best
Tomson