FluCalc

MSS-MLE (Ma-Sandri-Sarkar Maximum Likelihood Estimator) calculator for Luria–Delbrück fluctuation analysis.

Reference

Radchenko et. al, 2017 (Methods in Molecular Biology).
DOI: 10.1007/978-1-4939-7306-4_29

Installation

1. Clone or download source code.

2. Install miniconda

3. Create a new environment that uses Python 3.9 and all dependencies:

    conda create -n flucalc-env python=3.9
    conda activate flucalc-env
    pip install -r requirements.txt
   

4. Set FLUCALC_SECRET_KEY environment variable with a secret string. You can use any random sequence for it.

5. Change dir to the root of source code.

6. Start the server for FluCalc:

    gunicorn -w 4 -b <ip>:<port> flucalc:app
   

Or you can use start_server.sh script as alternative way to start the server:

  ./start_server.sh <ip>:<port>

6. For shutdown server press Ctrl + C in the terminal.