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Initial release#72
KateSakharova wants to merge 247 commits into
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dev

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@KateSakharova

@KateSakharova KateSakharova commented Jun 15, 2026

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nf-core/seqsubmit v1.0.0 — Initial Release

This is the first release of nf-core/seqsubmit, a Nextflow pipeline for submitting sequence data to the European Nucleotide Archive (ENA).

What it does:

The pipeline supports four submission modes via dedicated workflows:

  • mags — Metagenome Assembled Genomes submission (GENOMESUBMIT)
  • bins — Metagenomic bins submission (GENOMESUBMIT)
  • metagenomic_assemblies — Assembly submission (ASSEMBLYSUBMIT)
  • reads — Raw sequencing reads submission (READSUBMIT)

Key features:

  • Automatic study registration with ENA when no existing study accession is provided
  • Support for public and private data submissions, including configurable release dates
  • Coverage calculation from reads when not provided directly (via coverm)
  • Completeness/contamination estimation and taxonomy inference for MAGs/bins (via CheckM2 and CAT_pack) when metadata is incomplete
  • Credentials handled securely via Nextflow secrets (ENA_WEBIN, ENA_WEBIN_PASSWORD)
  • Full nf-core compatibility: runs with conda, Docker, and Singularity

Workflows and modules

  • READSUBMIT — manifest generation and webin-cli submission for raw reads
  • ASSEMBLYSUBMIT — manifest generation and webin-cli submission for metagenomic assemblies
  • GENOMESUBMIT — metadata table generation, genome upload, and webin-cli submission for MAGs/bins
  • Local modules: REGISTERSTUDY, CREATE_READS_MANIFEST, GENERATE_ASSEMBLY_MANIFEST, GENOME_UPLOAD, ENA_WEBIN_CLI_WRAPPER, and supporting utility modules

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github-actions Bot commented Jun 15, 2026

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nf-core pipelines lint overall result: Failed ❌

Posted for pipeline commit 6690021

+| ✅ 252 tests passed       |+
#| ❔   8 tests were ignored |#
!| ❗  15 tests had warnings |!
-| ❌   4 tests failed       |-
Details

❌ Test failures:

❗ Test warnings:

  • readme - README contains the placeholder zenodo.XXXXXXX. This should be replaced with the zenodo doi (after the first release).
  • pipeline_todos - TODO string in README.md: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file.
  • pipeline_todos - TODO string in README.md: Add bibliography of tools and data used in your pipeline
  • pipeline_todos - TODO string in nextflow.config: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
  • pipeline_todos - TODO string in nextflow.config: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
  • pipeline_todos - TODO string in CONTRIBUTING.md: Add any pipeline specific contribution guidelines here, such as coding styles, procedures, checklists etc.
  • pipeline_todos - TODO string in methods_description_template.yml: #Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline
  • pipeline_todos - TODO string in nextflow.config: Specify any additional parameters here
  • pipeline_todos - TODO string in test_full.config: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
  • pipeline_todos - TODO string in test_full.config: Give any required params for the test so that command line flags are not needed
  • pipeline_todos - TODO string in awsfulltest.yml: You can customise AWS full pipeline tests as required
  • pipeline_if_empty_null - ifEmpty(null) found in /home/runner/work/seqsubmit/seqsubmit/lint_results.md: _* pipeline_if_empty_null - No ifEmpty(null) strings found
    _
  • local_component_structure - rna_detection.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - fasta_validation.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - genome_evaluation.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure

❔ Tests ignored:

  • files_exist - File is ignored: conf/igenomes.config
  • files_exist - File is ignored: conf/igenomes_ignored.config
  • nextflow_config - Config variable ignored: params.input
  • files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md
  • files_unchanged - File ignored due to lint config: assets/nf-core-seqsubmit_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-seqsubmit_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-seqsubmit_logo_dark.png
  • container_configs - container_configs

✅ Tests passed:

Run details

  • nf-core/tools version 4.0.2
  • Run at 2026-06-25 19:25:17

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