Tri-Modal-Catheter-Lock-TCL

Tri-Modal Antifungal Catheter Lock (TCL): Proof of Invention Record

---

Tri-Modal Antifungal Catheter Lock (TCL)

Proof of Invention Record — Kapodistrian Academy of Science

This repository is permanently archived on Zenodo:
🔗 https://doi.org/10.5281/zenodo.17589267

---

📘 Overview

This repository contains the public disclosure and LaTeX documentation for the Tri-Modal Antifungal Catheter Lock (TCL) —
a novel, non-systemic antimicrobial lock formulation designed to eradicate Candidozyma (Candida) auris and other resistant fungal biofilms from indwelling medical devices.

The formulation integrates three synergistic components:

1. Echinocandin antifungal (e.g., micafungin)
2. β-1,3-glucanase enzyme (for extracellular matrix digestion)
3. Gallium(III)/EDTA complex (for iron substitution and metabolic disruption)

Together, these mechanisms collapse the biofilm matrix, inhibit cell-wall synthesis, and block iron metabolism — achieving ≥3-log CFU reduction without damaging catheter materials.

---

🧪 Scientific Context

• Inventor: Antonios Valamontes
• Affiliation: Kapodistrian Academy of Science
• Collaborating Witness: Dr. Ioannis Adamopoulos — Public Health and Environmental Inspector,
  Department of Environmental Hygiene and Public Health Inspections, Region of Attica, Hellenic Republic.
• Contact: [email protected]

This repository serves as an official timestamped record of invention for open scientific disclosure and non-commercial academic collaboration.

---

🔬 Scientific Summary

Mechanism of Action

Component	Target	Function
Echinocandin (micafungin)	β-glucan synthase	Disrupts fungal cell wall synthesis
β-1,3-Glucanase	Extracellular matrix	Liquefies polysaccharide ECM
Ga³⁺ + EDTA	Metal metabolism	Iron substitution & chelation

This tri-modal mechanism produces a superlinear reduction in residual viability:

$$ \Phi_{\mathrm{TCL}} = \eta_{\mathrm{ech}} \cdot \eta_{\mathrm{enz}} \cdot \left( 1 - \frac{[Fe^{3+}]_{\mathrm{biofilm}}}{[Fe^{3+}]_{0}} \right) $$

Where the combined inhibition terms result in complete matrix collapse and ≥3-log biofilm reduction within 90 minutes.

---

🧩 Repository Structure

📫 Contact

For collaboration, non-commercial research use, or commercial licensing discussions:

Antonios Valamontes
Kapodistrian Academy of Science
📧 [email protected]

---

🧾 Acknowledgment

Witnessed and independently verified by
Dr. Ioannis Adamopoulos,
Public Health and Environmental Inspector,
Department of Environmental Hygiene and Public Health Inspections,
Region of Attica, Hellenic Republic.

---

🧠 Keywords

Candida auris • biofilm • catheter lock • echinocandin • β-1,3-glucanase • gallium • EDTA • infection control • public health