Analyze Spheroid Cell Invasion In 3D Matrix

Analyze Spheroid Cell Invasion In 3D Matrix

The tool allows to measure the area of the invading spheroïd in a 3D cell invasion assay. You can find an example time-series of image here: lzrs1.zip.

Getting Started

The macro needs MorphoLibJ to be installed. In FIJI MorphoLibJ can be installed via the updater by activating the IJPB plugins site. To install the tools, drag the link: Analyze_Spheroid_Cell_Invasion_In_3D_Matrix.ijm to the ImageJ launcher window, save it under macros/toolsets in the FIJI installation and restart FIJI.

Select the Analyze_Spheroid_Cell_Invasion_In_3D_Matrix toolset from the >> button of the ImageJ launcher.

• the first button opens this help page
• the m button measures the area of the spheroid on the active image
• the b button runs the measurement of the spheroid in batch mode on a time-series of images

Options

By right-clicking on the m button you can open the options dialog.

• radius background subtraction: the rolling ball radius for the subtract background command
• remove small objects: if selected small objects will be removed from the result
• min. object size: the min. size of objects that will be taken into account when remove small objects is selected

Results

Publications using this tool

1. Van Treuren, T., and Vishwanatha, J.K. (2018). CRISPR deletion of MIEN1 in breast cancer cells. PLoS ONE 13, e0204976.

2. Sperlich, J., and Teusch, N. (2018). Pseudopterosin Inhibits Proliferation and 3D Invasion in Triple-Negative Breast Cancer by Agonizing Glucocorticoid Receptor Alpha. Molecules 23, 1992.