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Filament_Tools
        Volker edited this page Sep 22, 2025 
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    Calculate the area-fraction of the image filled with (septin) filaments.


To install the tool save the file filament_tools.ijm into the folder macros/toolsets of your FIJI installation.
Select the "filament tools" toolset from the >> button of the ImageJ launcher.
- press the a-button to analyze the active image
 - press the b-button to analyze the images in a folder in batch-mode.
 
Right-click the a-button to open the options dialog.
- filament channel - the number of the channel containing the filament image.
 - min. area - the minimal area an object must have to be considered a filament.
 - max. circularity - allows to remove objects too round to be filaments
 
Volker Bäcker