Incucyte_Exporter

Export the images from the Incucyte. Stitch the images, create a time-series and align the frames.

The source code in git-hub can be found here.

Getting started

You need to have the HyperStackReg plugin installed.

To install the tool save the file incucyte_exporter.ijm into the folder macros/toolsets of your FIJI installation.

Select the "incucyte_exporter" toolset from the >> button of the ImageJ launcher.

?

Open this help page.

export raw images as std. tif

Convert the images to a standard tif-format.

stitch images

Calculate the stitchings for the images.

clean images

Normalize the intensities and apply a rolling ball background subtraction.

merge images

Create the mosaic.

mark empty images

Prefixes the file name of the well-image with ``Empty_`` if no object has been found in the well.

make time series

Create a time series in one file from the different images of a well.

batch export images

Run all the before mentioned steps on the images.