MRI_Count_Spot_Populations_Tool
The tool detects and and counts the spots (or blobs) in an image. It has been created for the counting of bacteria colonies in in Petri-dishes. It separates the spots into two populations of different sizes and counts each population individually. It uses expectation maximisation clustering from the weka software.
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To install the tool save the files Count_Spot_Populations_Tool.ijm and results_table-clusterer.py into the folder macros/toolsets of your FIJI installation.
Select the "3D_nuclei_clustering" toolset from the >> button of the ImageJ launcher.
Volker Bäcker